THE SAGA COMPLEX IS ESSENTIAL FOR THE REGULATION OF GENES INVOLVED IN YEAST TREHALOSE METABOLISM

Saccharomyces cerevisiae accumulates trehalose as a stress metabolite in adverse environmental conditions. The synthesis and breakdown are important for the regulation of levels within yeast cell. Therefore, TPS1 NTH1 gene expressions tightly regulated during transcription also translation. Since both genes contain Stress Response Elements (STRE) promoter regions, they co-activated under However, presence similar regulatory elements shows that these undergo different at transcriptional level. In our study, role Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex was determined nutrient-poor environment. For purpose, wild type Δada1 mutant cells, where Ada1p is member SAGA complex, were grown normal nitrogen starvation addition, level detected enzymatically cells. silico analysis promoters revealed STRE sequences required binding Msn2/4 factors closed by nucleosomes promoter, but open promoter. absence Ada1p, stress-induced activation observed, while expression not activated. According to results, spanning could be mobilized Ada1 protein, therefore cannot bind activate It observed accumulation reduced regardless